Example 2

In this example, the full amount of required yield is not received before the yield timeout, requiring a lab requeue to request additional sequencing data

2a: Yield Requested

A biosample manifest is imported with a prep request required yield of 105 Gbp.

	Required	Expected	Actual	Pending
2a: Yield Requested	+105	+105	0	+105
Total	105	105	0	105

2b: Partial Yield Received

80 Gbp of QC-passed FASTQ data sets are received. The actual yield is increased and the amount still pending is changed to 15 GBP to reflect the remaining balance. The expected yield does not change because it represents the total amount expected from the lab and includes actual yield and pending yield.

	Required	Expected	Actual	Pending
2a: Yield Requested	+105	+105	0	+105
2b: Partial Yield Received			+80	–80
Total	105	105	80	25

2c: Yield Timeout

Yield timeout is reached with no additional yield received.

The default yield timeout is four days, however you can configure a different timeout period using the Basespace Sequence Hub API. For more information, see the developer documentation at developer.illumina.com.

When the yield timeout is reached, BaseSpace Sequence Hub assumes that the lab is unable to produce the requested quantity. The amount pending is classified as missing, and the expected yield is reduced to the actual yield received, indicating that no additional data are expected from the lab.

__

(table scrolls to the right)

	Required	Expected	Actual	Pending	Missing
2a: Yield Requested	+105	+105	0	+105	0
2b: Partial Yield Received			+80	–80	0
2c: Yield Timeout		-25		–25	+25
Total	105	80	80	0	25

2d: Lab Requeue for Additional Yield

A lab requeue for more yield is initiated. In this example, the user requests more than the missing amount.

• Expected yield updates. The updated amount reflects the quantity received from the initial request and the additional amount requested from the lab requeue. The required yield does not change because that is the amount of data required for analysis.

• Missing yield is reset to zero.

• Requested yield updates on the Requeues tab.

(table scrolls to the right)

	Required	Expected	Actual	Pending	Missing	Requested
2a: Yield Requested	+105	+105	0	+105	0
2b: Partial Yield Received			+80	–80	-
2c: Yield Timeout		-25		–25	+25
2d: Lab Requeue for Additional Yield		+30		+30	–25	+30
Total

2e: Balance of Yield Received

The lab produces 47 Gbp of QC-passed FASTQ data sets. Actual and expected yield are updated to reflect the total amount received from the lab.

(table scrolls to the right)

	Required	Expected	Actual	Pending	Missing	Requested
2a: Yield Requested	+105	+105	0	+105	0
2b: Partial Yield Received			+80	–80	-
2c: Yield Timeout		-25		–25	+25
2d: Lab Requeue for Additional Yield		+30		+30	–25	+30
2e: Balance of Yield Received		+17	+47	–30
Total	105	127	127	0	0	30