Plan a MiSeq i100 Run
Use the following instructions to plan a run for the MiSeq i100 Series systems in BaseSpace Sequence Hub.
Select the Runs tab, and then select the New Run drop-down.
Select Run Planning.
In the Run Name field, enter a unique name of your preference to identify the current run. The run name can contain a maximum of 255 alphanumeric characters, spaces, dashes, and underscores.
[Optional] Enter a description for the run. The run description can contain a maximum of 255 characters.
Select your sequencing system as the instrument platform.
Select one of the following analysis locations.
BaseSpace — Analyze sequencing data in the cloud.
Local — Analyze sequencing data on-instrument.
Enter the number of cycles performed in each read:
Read 1 — Enter the number of cycles for Read 1.
Index 1 — Enter the number of cycles for the Index 1 (i7) primer.
Index 2 — Enter the number of cycles for the Index 2 (i5) primer.
Read 2 — Enter the number of cycles for Read 2.
Select Next.
Select your analysis application. Please note that MiSeq i100 Series only supports one analysis per planned run.
[Optional] Enter a description for the configuration.
Select a library prep kit or add a new custom library prep kit as follows.
Select Add Custom Library Prep Kit under the Library Prep Kit dropdown.
Enter the name, read types, default read cycles, and compatible index adapter kits for your custom library prep kit.
Select Create New Kit.
Select an index adapter kit or add a new a custom index kits as follows. If you are using more than one library, the libraries must have the same index read lengths.
Select Add Custom Index Adapter Kit under the Index Adapter Kit dropdown.
Select a template type and enter the kit name, adapter sequences, index strategies, and index sequences. Make sure the second index (i5) adapter sequences are in forward orientation.
Select Create New Kit.
If applicable to your application, select a reference genome.
Select Next to configure secondary analysis settings.
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