Plan a NovaSeq X Series Run

Use the following instructions to plan a run for the NovaSeq X series systems in BaseSpace Sequence Hub.

  1. Select the Runs tab, and then select the New Run drop-down.

  2. Select Run Planning.

  3. In the Run Name field, enter a unique name of your preference to identify the current run. The run name can contain a maximum of 255 alphanumeric characters, spaces, dashes, and underscores.

  4. [Optional] Enter a description for the run. The run description can contain a maximum of 255 characters.

  5. Select your sequencing system as the instrument platform.

  6. Select one of the following analysis locations.

    • BaseSpace — Analyze sequencing data in the cloud.

    • Local — Analyze sequencing data on-instrument. When this option is selected, the planned run can only be exported to a sample sheet v2 file.

  7. If analyzing data locally, select one of the following FASTQ output format options: The output format is not available for cloud storage and only applicable if you select to keep FASTQ files when setting up secondary analysis.

    • gzip — Save the FASTQ files in gzip format.

    • DRAGEN — Save FASTQ files in ora format. See DRAGEN Compression for more details.

  8. Enter the number of cycles performed in each read: If using multiple analysis configurations, use the longest read length required by the configuration. When setting up a configuration, override automatically trims the length based on the recommended lengths for the selected library prep kit.

    • Read 1 — Enter up to 151 cycles.

    • Index 1 — Enter the number of cycles for the Index 1 (i7) primer. For a PhiX-only run, enter 0 in both index fields.

    • Index 2 — Enter the number of cycles for the Index 2 (i5) primer.

    • Read 2 — Enter up to 151 cycles. This value is typically the same as the Read 1 value.

  9. [Optional] Enter the ID for your library tube. The library tube ID is located on the label of your library tube strip.

  10. Select Next.

Please take note of the following when setting up a configuration.

  • Instrument Platform and Analysis location in Run Settings page are not editable once a Configuration is created.

  • Application version cannot be changed once a Configuration is saved. You need to delete the configuration and create a new one instead.

  1. Select your analysis application.

  2. [Optional] Enter a description for the configuration.

  3. Select a library prep kit or add a new custom library prep kit as follows.

    • Select Add Custom Library Prep Kit under the Library Prep Kit dropdown.

    • Enter the name, read types, default read cycles, and compatible index adapter kits for your custom library prep kit.

    • Select Create New Kit.

  4. Select an index adapter kit or add a new a custom index kits as follows. If you are using more than one library, the libraries must have the same index read lengths.

    • Select Add Custom Index Adapter Kit under the Index Adapter Kit dropdown.

    • Select a template type and enter the kit name, adapter sequences, index strategies, and index sequences. Make sure the second index (i5) adapter sequences are in forward orientation.

    • Select Create New Kit.

  5. If applicable to your application, select a reference genome.

  6. Select Next to configure secondary analysis settings.

Last updated