> For the complete documentation index, see [llms.txt](https://help.basespace.illumina.com/llms.txt). Markdown versions of documentation pages are available by appending `.md` to page URLs; this page is available as [Markdown](https://help.basespace.illumina.com/biosamples/biosample-workflow/add-prep-requests.md).

# Add Prep Requests

You can add new prep requests or update yield requirements for biosamples that have already been added to your account.

* To add new biosamples with prep requests, see [Add Biosamples](/biosamples/biosample-workflow/add-biosamples.md).
* To request more sequencing data without changing the yield target, see [Request a Lab Requeue](/automate/request-lab-requeue.md).

1. Create a CSV file as follows:
   1. Download or open a biosample workflow template.
   2. For each biosample, enter the following required information.
   * Biosample Name—The unique identifier for the source DNA.
   * Default Project—The default project for storing analysis data.
   * Prep Request—The name of the library prep kit to use for preparing the biosample. The name must exactly match an existing library prep kit.
   * Required Yield Gbp—The required yield for the prep request.
   3. \[Optional] Add instructions for analysis workflows.
   4. Save the file.
2. From the biosamples master list, select the File drop-down arrow, point to Upload, and then select Biosample Workflow.
3. In the Upload a Biosample Workflow dialog, add your biosample workflow file

{% hint style="info" %}
All template columns must be present in the biosample workflow file, but the Default Project cannot be changed using a biosample workflow. If the biosample does not exist in the specified project, BaseSpace Sequence Hub creates a new biosample in the project. For information about changing the default project for a biosample, see [Change Default Project](/biosamples/manage-biosamples.md#change-default-project).
{% endhint %}

BaseSpace Sequence Hub validates the file and identifies any errors.

* If there are errors in the manifest file, correct the errors and upload the file again.
* If there are no errors, review the notifications and select Continue.